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crab). The Horseshoe crabs are washed to remove sand and also other maritime particles from their exoskeletons; and those horseshoe crabs with no obvious accidents are placed on a bleeding rack and bled by puncturing the heart with a substantial gauge needle (Figure 3). On average, thirty % in the crab’s blood is eliminated prior to the wound clo
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Separation of mixtures is very influenced from the stream of cellular phase In the column [23, 24]. The stream amount is extremely essential in obtaining nicely-divided peaks without any tailing. The flow rate on the mobile phase could be optimized according to the retention time, column back pressure, and separation of closely eluting adjacent pea